Comparative study of four aortic allograft decellularization techniques

  • M. M. Shchatsinka Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
  • S. V. Spirydonau Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
  • V. A. Adzintsou Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
  • A. P. Shket Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
  • V. A. Yudzina City Clinical Pathologoanatomic Bureau, Minsk, Belarus
  • R. M. Smaliakova N. N. Alexandrov National Cancer Centre, Minsk, Belarus
  • A. I. Suboch N. N. Alexandrov National Cancer Centre, Minsk, Belarus
  • S. I. Dryk 9th City Clinical Hospital, Minsk, Belarus
  • M. K. Yurkshtovich Belarusian State University, Minsk, Belarus
  • Y. P. Ostrovsky Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
Keywords: aortic allograft, decellularization, tissue engineering

Abstract

Objective: to assess four aortic allograft decellularization techniques.

Materials and methods. 40 aortic allografts were decellularized using 4 techniques (n=10 in each group), depending on reagents used: 1) 1% deoxycholic acid; 2) 0,5% sodium deoxycholate, 0,5% sodium dodecylsulfate; 3) 1% sodium deoxy-cholate, 1% Triton X-100; 4) 3% Triton X-100, 0.04% EDTA. On decellularization completion, specimens were subjected to histologic (hematoxylin and eosin) and histochemic (MSB, orcein) studies; residual DNA content was assessed, as well as mechanical testing.

Results and discussion. Decellularization with 0.5% sodium deoxycholate, 0.5% sodium dodecylsulfate resulted in com-plete cell removal with good preservation of connective tissue matrix. Statistically significant reduction of DNA content in aorta (31,05 vs 2,58 ng/mg, p<0,01) and aortic valve leaflet (39,7 vs 1,36 ng/mg, p<0,05) was revealed. According to mechanical testing results, there were no significant differences between fresh, cryopreserved and decellularized allografts.

Conclusions. Complete allograft decellularization was achieved using 0.5% sodium deoxycholate, 0.5% sodium dodecy-lsulfate, with statistically significant DNA reduction and without affecting mechanical properties of the valve.

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Published
2017-05-15
How to Cite
Shchatsinka, M. M., Spirydonau, S. V., Adzintsou, V. A., Shket, A. P., Yudzina, V. A., Smaliakova, R. M., Suboch, A. I., Dryk, S. I., Yurkshtovich, M. K., & Ostrovsky, Y. P. (2017). Comparative study of four aortic allograft decellularization techniques. Ukrainian Journal of Cardiovascular Surgery, (1 (27), 62-69. Retrieved from http://cvs.org.ua/index.php/ujcvs/article/view/180
Section
ACQUIRED HEART DISEASES