Сравнительное исследование четырех методик девитализации аортальных аллографтов

M. M. Shchatsinka; S. V. Spirydonau; V. A.  Adzintsou; A. P. Shket; V. A. Yudzina; R. M. Smaliakova; A. I. Suboch; S. I. Dryk; M. K. Yurkshtovich; Y. P. Ostrovsky

M. M. Shchatsinka Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
S. V. Spirydonau Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
V. A. Adzintsou Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
A. P. Shket Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus
V. A. Yudzina City Clinical Pathologoanatomic Bureau, Minsk, Belarus
R. M. Smaliakova N. N. Alexandrov National Cancer Centre, Minsk, Belarus
A. I. Suboch N. N. Alexandrov National Cancer Centre, Minsk, Belarus
S. I. Dryk 9th City Clinical Hospital, Minsk, Belarus
M. K. Yurkshtovich Belarusian State University, Minsk, Belarus
Y. P. Ostrovsky Republican Scientific and Practical Center “Cardiology”, Minsk, Belarus

Keywords: aortic allograft, decellularization, tissue engineering

Abstract

Objective: to assess four aortic allograft decellularization techniques.

Materials and methods. 40 aortic allografts were decellularized using 4 techniques (n=10 in each group), depending on reagents used: 1) 1% deoxycholic acid; 2) 0,5% sodium deoxycholate, 0,5% sodium dodecylsulfate; 3) 1% sodium deoxy-cholate, 1% Triton X-100; 4) 3% Triton X-100, 0.04% EDTA. On decellularization completion, specimens were subjected to histologic (hematoxylin and eosin) and histochemic (MSB, orcein) studies; residual DNA content was assessed, as well as mechanical testing.

Results and discussion. Decellularization with 0.5% sodium deoxycholate, 0.5% sodium dodecylsulfate resulted in com-plete cell removal with good preservation of connective tissue matrix. Statistically significant reduction of DNA content in aorta (31,05 vs 2,58 ng/mg, p<0,01) and aortic valve leaflet (39,7 vs 1,36 ng/mg, p<0,05) was revealed. According to mechanical testing results, there were no significant differences between fresh, cryopreserved and decellularized allografts.

Conclusions. Complete allograft decellularization was achieved using 0.5% sodium deoxycholate, 0.5% sodium dodecy-lsulfate, with statistically significant DNA reduction and without affecting mechanical properties of the valve.

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Comparative study of four aortic allograft decellularization techniques

Abstract

References