@article{Shchatsinka_Spirydonau_Adzintsou_Shket_Yudzina_Smaliakova_Suboch_Dryk_Yurkshtovich_Ostrovsky_2017, title={Comparative study of four aortic allograft decellularization techniques}, url={https://cvs.org.ua/index.php/ujcvs/article/view/180}, abstractNote={<p><strong>Objective</strong>: to assess four aortic allograft decellularization techniques.</p> <p><strong>Materials and methods</strong>. 40 aortic allografts were decellularized using 4 techniques (n=10 in each group), depending on reagents used: 1) 1% deoxycholic acid; 2) 0,5% sodium deoxycholate, 0,5% sodium dodecylsulfate; 3) 1% sodium deoxy-cholate, 1% Triton X-100; 4) 3% Triton X-100, 0.04% EDTA. On decellularization completion, specimens were subjected to histologic (hematoxylin and eosin) and histochemic (MSB, orcein) studies; residual DNA content was assessed, as well as mechanical testing.</p> <p><strong>Results and discussion</strong>. Decellularization with 0.5% sodium deoxycholate, 0.5% sodium dodecylsulfate resulted in com-plete cell removal with good preservation of connective tissue matrix. Statistically significant reduction of DNA content in aorta (31,05 vs 2,58 ng/mg, p&lt;0,01) and aortic valve leaflet (39,7 vs 1,36 ng/mg, p&lt;0,05) was revealed. According to mechanical testing results, there were no significant differences between fresh, cryopreserved and decellularized allografts.</p> <p><strong>Conclusions</strong>. Complete allograft decellularization was achieved using 0.5% sodium deoxycholate, 0.5% sodium dodecy-lsulfate, with statistically significant DNA reduction and without affecting mechanical properties of the valve.</p&gt;}, number={1 (27)}, journal={Ukrainian Journal of Cardiovascular Surgery}, author={Shchatsinka, M. M. and Spirydonau, S. V. and Adzintsou, V. A. and Shket, A. P. and Yudzina, V. A. and Smaliakova, R. M. and Suboch, A. I. and Dryk, S. I. and Yurkshtovich, M. K. and Ostrovsky, Y. P.}, year={2017}, month={May}, pages={62-69} }